Protein sequencing: a practical approach

A practical approach to a low protein diet in Brazil

BACKGROUND Chronic kidney disease (CKD) is an emerging health public problem in Brazil. Nutritional counseling with focus on protein restriction is a promising strategy to treatment of nondialysis CKD patients due its effects on slowing renal loss. However, Brazilian people have high protein intake, which is a challenge when low protein diet (LPD) should be prescribed. This review describes a p...

A Practical Approach on SDS PAGE for Separation of Protein

Polyacrylamide gel electrophoresis (PAGE), describes a technique widely used in biochemistry, forensics, genetics, molecular biology and biotechnology to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility. Mobility is a function of the length, conformation and charge of the molecule. As with all forms of gel electrophoresis, molecu...

Metaheuristics based de novo protein sequencing: A new approach

In this article, a new approach is proposed for the de novo protein sequencing problem. The aim is to find the sequence of an experimental protein from only experimental data i.e. without databases. To do so, a three-step model called SSO for Shape, Sequence and Order has been designed. No prior knowledge in genomics nor protein databases are used. Here we modelize de novo protein sequencing as...

A Practical Approach

Background: Diplopia is a common complaint in both inpatient and outpatient neurologic practice. Its causes are many, and special historical and examination features are important to localization and accurate diagnosis. Review Summary: This review is divided into 2 sections: the first related to diagnosis and the second to treatment of binocular diplopia. In the diagnostic section, emphasis is ...

A practical approach